Flox

FLUORESCENCE BOX

The fluorescence box (FloX) is an unique instrument, enabling the continous observation of chlorophyll fluorescence. It measures spectral data of extremely high resolution, yet is built to withstand the hazards of a permanent outdoor installation.

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DESCRIPTION

The fluorescence box (FloX) is an unique instrument, enabling the continous observation of chlorophyll fluorescence. It measures spectral data of extremely high resolution, yet is built to withstand the hazards of a permanent outdoor installation.

It represents the evolution of prototypes such as the Multiplexer Radiometer Irradiometer (MRI), SFLUOR box and SIF-System developed from a collaboration between Jülich Research Center and the Remote Sensing of Environmental Dynamics Laboratory of the University Milano Bicocca. The basic routines of the FloX are based on SPECY (Forschungszentrum Jülich, IBG-2: Plant Sciences).

The FloX is specifically designed to passively measure chlorophyll fluorescence under natural light conditions. Therefore the design is optimized to achieve maximum efficiency in terms of: Signal to Noise Ratio, Spectral Resolution and quick acquisition time. The core of the system is the QEPro spectrometer from Ocean Optics covering the Red/Near Infrared region (650 – 800 nm). This is the spectral range where chlorophyll fluorescence is emitted and where the two atmospheric oxygen absorption bands (O2B and O2A, at 689 nm and 760 nm respectively) are used to measure it. Upward and downward channels of the FloX allow to measure the solar irradiance and the reflected radiance from the canopy. In order to keep a stable level of dark current the spectrometers are embedded in a temperature controlled housing. The signal-to-noise is maximized thanks to accurate automatic optimization of the signal of both channels and a high performance optical light throughput.

Specifications

OPTIC Spec 1

Wavelength range

650—800 nm

Spectral Sampling Interval (SSI)

0.17 nm

Spectral resolution (FWHM)

0.3 nm

Signal to Noise Ratio (SNR)

1000

Field Of View (FOV)

Dual FOV. Upwelling radiance 25°. Downwelling radiance 180°

OPTIC Spec 2

Wavelength range

~ 400–950 mn

Spectral Sampling Interval (SSI)

~ 0.65 nm

Spectral resolution (FWHM)

~ 1.5 nm

Field Of View (FOV)

Dual FOV. Upwelling radiance 25°. Downwelling radiance 180°

OPERATIONAL

Signal Optimization

Automatic adaption to varying light conditions

Dark current

Accurate dark current determination at each measurement cycle

Manual acquisition

Interface software for manual measurement and calibration

Automatic acquisition

Fully autonomous measurement mode for unattended data acquisition

Quick measurements

20 seconds under bright sunshine 60 seconds in overcast condition

Stability

Reference system stability check and uncertainty estimates

Simultaneous metadata

Spectrometer temperature, Outside temperature, GPS position, GPS time

Data Display

Live assessment of the systems status

Data storage

SD card up to 32 GB (12 months of measurements)

Case

Robust and Waterproof housing based on the 1510 Pelicase

Dimension

Small form factor (50 × 30 × 20 cm)

Power supply

12 Volt. From battery or solar panels

Power consumption

Average consumption of 60 Watt. (20/100 Watt, cooling on/off)

Energy saver

Day/night switch for energy saving

Interfaces

RS232 via cable and wireless

OPTIONAL

Dust Protection

Additional dust protection for Cosine Receptors

Fiber optics

Flexible length of fiber optics according to user needs

Power supply

Solar panel and battery

Communication

Addon for LAN/WLAN/Mobile Network Remote access

VIDEO TUTORIAL

FLOX IN ACTION

FAQ

How do I process the FloX data?

To calculate fluorescence, radiance, reflectance and a variety of vegetation indices, an open source R package was made in collaboration with Max Planck Institute and University of Milano Bicocca and is available in github. The package is wrapped with a graphical user interface to allow processing of years of data with a few clicks.

How can the FloX be integrated for remote access and live datastream

The basic FloX is equipped with an USB interface to stream data directly to a connected computer. Second a Xbee based wireless receiver comes with the system to receive data from a distance. If thats not enough, we can support turn-key solutions for remote control via ethernet, wireless LAN or even mobile network.

Can the FloX be used on a drone?

Don´t even think of it. The FloX is a rugged and pretty heavy instrument and absolutely not designed for airborne use. But you may get in touch with us for a glimpse on some secret projects.

Can the FloX be used in a mobile way?

Yes. Checkout the FloX2Go option but be aware that fluorescence measurements require very stable measurement setups and work best in a fixed position.

What is the delivery time of the instrument?

Delivery time ranges from one to a maximum of three month

Can JB provide assistance for instrument installation?

Yes. Just get in touch with us to request our installation service.

PUBLICATIONS

An R Package for Field Spectroscopy: From System Characterization to Sun-Induced Chlorophyll Fluorescence Retrieval. 17-19 January 2017, ESA ESRIN, Frascati (Rome), Italy.

Julitta T. Wutzler T. Rossini M. Colombo R. Cogliati S. Meroni M., Burkart A. Migliavacca M.

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Accurate measurements of fluorescence in the O2A and O2B band using the FloX spectroscopy system – results and prospects. Potsdam Greenhouse Gas Workshop, From Photosystems to Ecosystems 24 – 26 October 2017.

Julitta T. Burkart A. Colombo R. Rossini M. Schickling A. Migliavacca M. Cogliati S. Wutzler T. Rascher U.

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Characterisation of reflectance and chlorophyll fluorescence anisotropy – defining requirements for an experimental setup. Geophysical Research Abstracts, vol. 20, EGU2018-16936, 2018. EGU General Assembly 2018

Biriukova K. Julitta T. Celesti M. Panigada C. Evdokimov A. Migliavacca M. Rossini M

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Exploiting top of Canopy Sun Induced Chlorophyll Fluorescence by the FloX. From Instrument performance to data processing. OPTIMISE COST Action Final Meeting. 21-23 February 2018, Sofia (Bulgaria)

Julitta T. Burkart A. Colombo R. Rossini M. Schickling A. Migliavacca M. Cogliati S. Wutzler T. Näthe P. Rascher U.

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